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Previous Work

Optical Recordings of Intracellular pH in Tadpole Brainstems

We used tadpole brainstems to investigat pH change in chemosensitive respiratory neurons in order to better understand the fundamental mechanisms of respiration. This was done in order to gain insight into the pathology of Sudden Infant Death Syndrome (SIDS).

We employed pH-sensitive, ratiometric fluorophores to make real time, optical recordings of intracellular pH from living tissue. We used the pH-sensitive dye BCECF to record intracellular pH from central respiratory chemoreceptors of the developing tadpole brainstem.


BCECF emitted fluorescence from the tadpole brainstem (10X)

 


BCECF emitted fluorescence from the tadpole brainstem (40X)

 

We also investigated the impact of both caffeine and serotonin on respiratory behavior using the adenosine antagonist DPCPX and the serotonin receptor agonist 5-HT.