Fees

Fees subject to revision without notice

Single-Cell Services

10X Genomics

library prep only; sequencing is separate
capture up to 10,000 cells/sample
recommended sequencing 25,000-50,000 reads/cell (depending on application)

Single Cell 3' Gene Expression (Library Prep)

	UT System	Non-UT System
1st Sample	$2330	$3484
Each Concurrent Sample up to 8	$1662	$2380

Single Cell ATAC-Seq (Library Prep)

	UT System	Non-UT System
1st Sample	$1972	$2949
Each Concurrent Sample up to 8	$1452	$2170

Milo Single-Cell Western

capture up to 1000 cells per sample, one sample per chip
sample can be stored for 9 months and re-probed multiple times
analysis software, Scout, is free to download (follow link to download: http://proteinsimple.com/scout/downloads/)

	UT System	Non-UT System
Loading Chip (1 sample/chip)	$212	$317
Probing & Scanning (2 samples/run)	$171	$256
Scout Analysis (per hour)	$42	$63

Fluidigm C1 Single-Cell Auto-Prep System

	UT System and Non-UT System
DNAseq	please inquire
RNAseq	please inquire
qPCR for Single-Cell	please inquire
Custom Application (open app)	please inquire

Next Generation Sequencing

Library Preparation

For custom library prep needs, please inquire

	UT System	Non-UT System
Nextera XT (10 samples)	$66/sample	$99/sample
QuantSeq mRNAseq (12 samples)	$66/sample	$99/sample
16S Library Prep (16 samples)	$29/sample	$44/sample
Amplicon Library Prep (12 samples)	$30/sample	$45/sample

MiSeq Sequencing Runs

reads per run varies and is dependent on library type

MiSeq Reagent v3 Kits (~25M reads/run)

	UT System	Non-UT System	Applications
75bp Paired End (150 cycles)	$1131	$1691	RNAseq, small RNAseq
300bp Paired End (600 cycles)	$1796	$2686	WGS

MiSeq Reagent v2 Kits (~15M reads/run)

	UT System	Non-UT System	Applications
50bp Single End (50 cycles)	$1036	$1549	small RNAseq
150bp Paired End (300 cycles)	$1281	$1916	targeted sequencing
250bp Paired End (500 cycles)	$1416	$2117	WGS

MiSeq Library QC Reagent Kits

ideal for running QC on libraries before deep sequencing

	UT System	Non-UT System	Reads/Run (Millions)
150bp Paired End (300 cycles)	$636	$951	4
150bp Paired End (300 cycles)	$481	$720	1
250bp Paired End (500 cycles)	$541	$809	1

BioMarkHD High-Throughput qPCR Services

High-Throughput qPCR

format of chips is #samples x #assays

	UT System	Non-UT System
Reverse Transcription (24 samples)	$147	$220
PreAmp Services (24 samples)	$179	$268
Delta Gene Assays
FlexSix 12x12 – EvaGreen	$215	$322
48x48 – EvaGreen	$636	$951
96x96 – EvaGreen	$1370	$2049
192x24 – EvaGreen	$1229	$1838
TaqMan Assays
FlexSix 12x12 – TaqMan	$250	$374
48x48 – TaqMan	$657	$983
96x96 – TaqMan	$1372	$2052
192x24 – TaqMan	$1146	$1714

High-Throughput SNP Genotyping

	UT System	Non-UT System
FlexSix 12x12 – TaqMan	$183	$274
48x48 – TaqMan	$706	$1056
96x96 – TaqMan	$988	$1478
192x24 – TaqMan	$881	$1318

Digital PCR Services

	UT System	Non-UT System
12.765 Digital GE Chip – TaqMan	$706	$1056
48.770 Digital GE Chip – TaqMan	$1221	$1826
37k Digital Chip – TaqMan	$375	$561

Sample QC & Ancillary Instruments

Sample QC

	UT System	Non-UT System
PicoGreen dsDNA Assay (24 samples)	$110	$165
Bioanalyzer 6000 Nano RNA Chip	$47	$70
Bioanalyzer High Sensitivity DNA Chip	$103	$151
TapeStation D1000 TapeScreen	$79	$119
TapeStaiton D5000 TapeScreen	$107	$160
Qubit DNA/RNA (per sample)	$4	$6

Ancillary Instruments (UTSA Labs Only)

	Instrument Only	Reagents
QuantStudio5 qPCR System (per hour)	$23	$8
Bioruptor Run (per run)	$20	n/a
Synergy II Run (per run)	$35	$69 (24 samples)
Bioanalyzer Run (per run)	$19	$54 (RNA)	$81 (DNA)
TapeStation (per run)	$4	$6/sample
Countess II FL (per sample)	n/a	$3
Qubit 2.0	n/a	$2

Acknowledgements

Each publication, press release, or other documentation must acknowledge research supported by the UTSA Genomics Core with the following statement:
Results were generated in part with help from the UTSA Genomics Core which is supported by NIH grant G12-MD007591, NSF grant DBI-1337513, and UTSA.