Gary Gaufo, Ph.D.
Ph.D.; University of California, Berkeley
M.A.; University of California, Berkeley
B.A.; University of California, Berkeley
Dr. Guafo’s lab is interested in understanding how evolutionarily conserved patterning programs along the anteroposterior (AP) and dorsoventral (DV) axes are integrated to generate functional and cellular complexity in vertebrates. By tinkering with these axial patterning programs, the lab hopes to learn about converging mechanisms that control progenitor or stem cell fate, and ultimately how this information may provide insight into altering the course of diseased cells.
Dr. Guafo's lab uses a multi-pronged approach to investigate the mechanisms that generate functional and cellular complexity in the vertebrate head. The primary research tool is the genetically-engineered mouse. The lab uses various mouse lines harboring conditional and mutant alleles for a myriad of transcription factors and signaling molecules, Cre, and inducible-Cre recombinases, and GFP reporters.
To analyze the phenotypes associated with genetically manipulated mice, the lab uses confocal microscopy, microcomputed tomography, basic histology, the neurosphere assay, and basic biochemical and genomic assays, to name just a few. To complement their in vivo approach, thelab uses the CRISPR/Cas9 system to manipulate gene expression in induced pluripotent stem cells to understand the molecular mechanisms controlling differentiation of neural progenitor and osteoprogenitor cells.
Click here for a list of publications.