John R. McCarrey, Ph.D.
Areas of Specialization
» Epigenetic regulation of cell functions
» Mammalian germ cells and stem cells
Ph.D. in Genetics; University of California, Davis
M.S. in Genetics; University of California, Davis
B.S. in Animal Science; University of California, Davis
Research in Dr. McCarrey’s lab is centered on the development, differentiation, and epigenetic regulation of mammalian germ cells and stem cells. The lab is interested in 1) the occurrence and correction of epimutations in the germline, 2) the effects of assisted reproductive technologies on the induction of epimutations in the offspring produced, 3) the development of spermatogonial stem cells, 4) the maintenance of enhanced genetic integrity in germline and pluripotent cells, 5) the regulation of gene expression in germ cells, 6) X-chromosome activity or inactivity in germ cells, 7) germline epigenetic programming, and 8) developing the baboon as a preclinical model for studies of stem cell-based therapies.
Opportunities exist for training in association with any of the research foci listed above. Technical approaches include analysis of gene expression patterns by RNA-seq; molecular regulation of gene expression, including analysis of protein-DNA interactions by ChIP; DNA methylation by methyl-DIP-seq; histone modifications by ChIP-seq; chromatin structure by ATAC-seq; derivation and culture of pluripotent stem cells and germ cells, particularly spermatogonial stem cells; analysis of mutation frequencies; stem-cell based therapeutic approaches via derivation and directed differentiation of iPS cells and transplantation into mice or nonhuman primates.
Click here for a list of publications.