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Faculty
     
  Harry Wellington Jarrett III
Professor

Office Phone: (210) 458-7053
Office: BSE 1.104G
E-Mail: harry.jarrett@utsa.edu

Areas of Specialization
• Electrochemical (Bio)sensors
• Functional Electrode Layers
• Inorganic Electrocatalysts





Research Interests


We contribute to two fields:


• Signal transduction, and more specifically the role of Ca2+ and its receptor protein, calmodulin, in cellular regulation. We have showed that dystrophin, the protein product of the Duchenne muscular dystrophy gene locus, is a specific calmodulin-binding protein and discovered a signal transduction pathway, which links laminin-α-dystroglycan-β-dystroglycan-
dystrophin-syntrophin-Grb2-Sos1-Rac1-PAK1-MKK4/7-JNK, causing c-jun phosphorylation and activation. This work is supported by National Institutes of Health grant 1R01AR051440.


• Transcription factor purification and characterization: We first coupled DNA to HPLC silica and have made a significant contribution to the purification and characterization of transcription factors. Most recently we developed systematic oligonucleotide trapping, a method for purifying transcription factors to near homogeneity in a single step. Current experiments involve promoter trapping, a method which interfaces DNA-HPLC with proteomic techniques (MALDI-TOF and ESE-ion trap) to characterize the transcription complex of the c-jun promoter. This work is supported by National Institutes of Health grant 1R01GM43609.




Selected Publications

 

Laminin-α1 LG4-5 domain binding to dystroglycan mediates muscle cell survival, growth, and the AP1 and NF-κB transcription factors but also has adverse effects. 
Zhou, Y.W., Munoz, J., Jiang, D., and Jarrett, H.W
Am. J. Physiol.Cell.Physiol.302: C902-14, 2012.

 

Transcription Factor Proteomics: Identification by a Novel Gel Mobility Shift-Three-Dimensional Electrophoresis Method Coupled with Southwestern Blot and HPLC-Electrospray Mass Spectrometry Analysis.
Jiang, D., Jia, Y., and Jarrett, H.W.
J. Chromatogr. A 1218: 7003-7015, 2011.

 

 

Purification and identification of a transcription factor, USF-2, binding to E-box element in the promoter of human telomerase reverse transcriptase (hTERT).
Jiang, S., Galindo, M.R., and Jarrett, H.W.
Proteomics 10: 203-211, (PMID: 19899074), 2010.

 

 

LG4-5 domains of laminin-2 binds a-dystroglycan to allow myotube attachment and prevent anoikis.
Munoz, J., Zhou, Y., and Jarrett, H.W.
J. Cell Physiol. 222: 111-119, (PMID: 19739104), 2010.

 

 

An insertion of an intracisternal type A particle retrotransposon in a novel member of the phosphoglycerate mutase family in the lew allele of mutant mice.
Jiao, Y., Jin, X., Yan, J., Jiao, F., Li, X., Roe, B.A., Jarrett, H.W., Gu, W.
Genes Genet. Syst. 8: 327-334, (PMID: 20154419), 2009.

 

 

Methods for Proteomic Analysis of Transcription Factors.
Jiang, D., Jarrett, H.W. and Haskins, W.E.
J. Chromatogr. A, 1216: 6881-6889, (PMID: 19726046), 2009.

 

 

Two-Dimensional Southwestern Blotting and Characterization of Transcription Factors On-blot.
Jiang, D. Jia, Y., Zhou, Y. and Jarrett, H.W.
J.Proteome Res. 8: 3693-3701 (PMID 19388704), 2009.

 

 

Dystrophin Glycoprotein Complex-associated Gβγ Subunits Activate Phosphatidylinositol-3-Kinase/Akt signaling in Skeletal Muscle in a Laminin-dependent Manner.
Xiong, Y., Zhou, Y.W., and Jarrett, H.W.
J. Cell Physiol, 219: 402-414 (PMID: 19117013), 2009.

 

 

Two-Dimensional Southwestern Blotting and Characterization of Transcription Factors On-blot.
Jiang, D. Jia, Y., Zhou, Y. and Jarrett, H.W.
J.Proteome Res., in press, 2009

 

 

Purification and identification of positive regulators binding to a novel element in the c-jun promoter.
Jiang, D., Zhou, Y.W., Moxley, R.A., and Jarrett, H.W.
Biochemistry 47: 9318-9334, 2008.

 

 

 

TRAPPING OF TRANSCRIPTION FACTORS WITH SYMMETRICAL DNA USING THIOL-DISULFIDE EXCHANGE CHEMISTRY.
PANDA, M., JIANG, D., AND JARRETT, H.W.
J. CHROMATOGR. A 1202: 75-82, 2008

 

 

Purification and identification of transcription factors with mass spectrometry.
Jiang, S., Nagore, L., Jiang, D., Moxley, R.A., and Jarrett, H.W.
Current Analytical Chemistry 3: 272-282, 2007.

 

 

Laminin-induced activation of Rac1 and JNKp46 is initiated by Src family kinases and mimics the effects of skeletal muscle contraction.
Zhou, Y.W., Thomason, D.B. and Jarrett, H.W.
Biochemistry 46: 14907-16, 2007.

 

 

Binding of laminin α1-chain LG4-5 domain to α-dystroglycan causes tyrosine phosphorylation of syntrophin to initiate Rac1 signaling.
Zhou, Y.W., Thomason, D.B., Gullberg, D., and Jarrett, H.W.
Biochemistry 45: 2042-52, 2006.

 

 

Promoter trapping of c-jun promoter-binding transcription factors.
Jiang, D., Moxley, R.A., and Jarrett, H.W.
J. Chromatogr. A. 1133: 83-94, 2006.

 

 

Cyanogen Bromide Activated Coupling - DNA Catalytic Chromatography Purification of EcoRI Endonuclease.
Nagore, L.I., Mitra, S., Jiang, D., Jiang, S., Zhou, Y.W., Loranc, M., and Jarrett, H.W.
Nature Protocols 1: 2909-15, published online, 2006.

 

 

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