Instrumentation


Major Equipment

Drop-Seq
10X Genomics Chromium Single-cell (Drop-Seq)
  • Single-cell RNA-seq of 500 to 10,000 cells for 1-8 samples at a time
  • Digital cell and transcript barcoding
  • Up to 55% capture efficiency for rare cells
  • Fast runs (6.5 min) and sequencing-ready libraries in 1-2 days

 

Fluidigm C1
Fluidigm C1
  • Single-cell nucleic acid prep system for interrogating genomes at the single cellular level
  • cDNA ~ RNA-Seq/transcriptome
  • gDNA ~ genome sequencing
  • Chips can accommodate cells between 5-25 microns, typical eukaryotic cell sizes

 

Biomark HD/C1 System
Biomark HD/C1 System
  • BioMark Integrated Fluidic Circuit (IFC)
  • High-throughput real time qPCR
  • Multiplex either 48.48 or 96.96 ~ gene targets.samples

 

MiSeq NGS
MiSeq NGS
  • Whole genome sequencing of small genomes
  • Targeted sequencing of large genomes
  • Shorter run time than HiSeq

 

Other Equipment

The Genomics Core also maintains the following equipment for assisted or unassisted use:

  • 2100 BioAnalyzer (Agilent)
  • BioRuptor (Diagenode)
  • Ion Torrent PGM (ThermoFisher Scientific)
  • PX1 Plate Sealer (Bio-Rad)
  • Quant Studio 5 qPCR (ThermoFisher Scientific)
  • Synergy 2 fluorescence/absorbance microtiter plate reader (BioTek)

Contact the core director or manager for details.

User Support Services

Our core provides the following services:

  • Preparation of libraries from genomic DNA for whole genome sequencing
  • Preparation of transcriptome libraries from single and bulk cell cDNA